Analysis of Microbial Abundances in Biofilms and Water in Hypersaline Environments with Different NaCl Levels

Biofilm is the dominant habitat for aquatic microbes that can grow in various conditions, including environments with extreme conditions. One of conditions found ecosystems a hypersaline environment. As part biological resources, also live this However, although it predominant microbes, biofilms have yet to be widely explored. Understanding environments, especially salt ponds, will increase knowledge about microbial biofilms. The open opportunities using as such aquaculture or environmental biotechnology. This study analyzed abundance and water environments. from ponds on South Coast Malang Regency, Indonesia, different salinity levels (2%, 25%, 40%). Microbial was culture method several media (Modified Nutrient Agar, Modified Total Plate Mannitol Salt Casein Medium Agar). substrate biofilm growth HDPE, often used bottom coating shrimp ponds. Moreover, quality parameters (pH, dissolved oxygen, turbidity, conductivity, total solids, temperature) during biofilms' forming process. results indicate good around strongly influenced by type culturing. main composition most influential peptone. density hundreds thousands times higher than surrounding water. show presence both affects properties Keywords: ecosystem, biofilm, hypersaline, ecology.